NAD+ 500mg

Nicotinamide adenine dinucleotide (NAD+) is a critical, ubiquitous coenzyme present in all living cells, serving as a principal electron carrier in redox reactions and a central hub of cellular metabolism.

In its oxidized form (NAD+), it acts as an essential co-substrate for key enzyme families—including sirtuins (SIRT1–7), poly(ADP-ribose) polymerases (PARPs), and CD38/CD157 ectoenzymes—linking its bioavailability to core processes such as energy metabolism, DNA repair, circadian rhythm regulation, and cellular stress response. Research-grade NAD+ is supplied as a high-purity, lyophilized or crystalline powder (typically β-NAD+, disodium salt) for controlled laboratory investigation into age-related NAD+ decline, mitochondrial dysfunction, and interventions targeting the NAD+/NADH ratio. This compound is intended strictly for in vitro and non-human in vivo research to elucidate biochemical pathways governing cellular aging, metabolic disease, and neuroprotection.

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Complete Research Kit Contents & Verification

Each NAD+ order ships as a comprehensive experimental package designed for sensitive biochemical and metabolic research:

• Primary Research Compound:
  10 sterile glass vials, each containing 500 mg of high-purity β-NAD+ (disodium salt) powder (total 5 g).

• Reconstitution Solution:
  30 mL vial of sterile 0.9% Sodium Chloride (saline) or a specialized NAD+ stabilization buffer.
  Note: Bacteriostatic water is not recommended for NAD+ due to potential degradation from benzyl alcohol; sterile saline is provided instead.

• Laboratory Administration Tools:
  Pack of sterile syringes (1 mL for precise dosing and 3 mL for reconstitution) plus alcohol swabs for aseptic handling.

• Research Protocol Documentation:

  • Dosing Schedule: Protocol for research models, including common concentrations for cell culture supplementation (e.g., 1–10 mM) and key considerations for in vivo studies.

  • Usage Instructions: Critical handling guide covering light- and temperature-sensitive procedures, rapid reconstitution with cold solution to minimize degradation, short-term storage guidelines (aliquot and store at −80 °C), and compatibility notes for various assay systems.

• Quality Verification – Certificate of Analysis (CoA) per Batch:

  • Purity analysis: >98% by HPLC (assessing NAD+ vs. degradation products such as NADH, ADP-ribose, nicotinamide)

  • Identity confirmation: UV-Vis spectroscopy (characteristic peak ~259 nm; A250/A260 ratio)

  • Potency/content assay: enzymatic cycling assay

  • Sterility testing (for solutions intended for in vivo research)

  • Endotoxin levels: <0.1 EU/mg (suitable for sensitive cell work)

  • Water content (for lyophilized form)

  • pH of reconstituted solution

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Research Applications & Scientific Utility

In laboratory environments, research-grade NAD+ enables investigation into foundational biochemical and metabolic pathways:

• Sirtuin Research:
  Study of SIRT1–7 activation and their roles in deacetylation, metabolic regulation, stress resistance, and longevity-associated signaling.

• Mitochondrial Function:
  Evaluation of electron transport chain efficiency, ATP production, mitochondrial biogenesis, and NAD+-dependent metabolic flux.

• Aging & Senescence Models:
  Research into NAD+ depletion as a hallmark of aging and testing of NAD+ repletion strategies in cellular and animal models.

• DNA Repair Studies:
  Examination of PARP-mediated DNA repair mechanisms in response to genotoxic stress and their dependence on NAD+ availability.

• Metabolic Disease Research:
  Exploration of NAD+ metabolism in models of obesity, type 2 diabetes, non-alcoholic fatty liver disease (NAFLD), and related metabolic disorders.

• Neurodegeneration Research:
  Investigation of NAD+’s role in neuronal health, bioenergetics, and resilience in models of Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative conditions.

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Laboratory Handling & Safety Protocol – CRITICAL

• Storage:
  Store unopened vials at −20 °C or, ideally, −80 °C, protected from light and moisture. Stability is paramount. After reconstitution, aliquot immediately and store at −80 °C. Avoid repeated freeze–thaw cycles.

• Reconstitution:
  Use ice-cold, sterile saline or the specified stabilization buffer. Work quickly on ice. Gently swirl to dissolve—do not vortex. The solution is highly unstable at room temperature and neutral pH.

• Light Sensitivity:
  Perform all handling under low-light conditions. Use amber vials or wrap containers in foil for storage to prevent photodegradation.

• Laboratory Safety:
  Standard PPE (lab coat, nitrile gloves, safety glasses) is required. While NAD+ is not highly toxic, it should be treated as a potent biochemical reagent and handled according to institutional safety SOPs.

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Critical Compliance & Intended Use Disclaimer

THIS PRODUCT IS SOLD FOR RESEARCH USE ONLY (RUO). NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.

• Intended Use:
  NAD+ is supplied strictly as a biochemical reagent for laboratory research. It is intended for use by qualified researchers in controlled in vitro or non-human in vivo settings.

• Not for Human Consumption:
  Under no circumstances is this product intended for intravenous, subcutaneous, or any form of human administration. It is not a drug, anti-aging supplement, or IV therapy ingredient.

• Regulatory Status:
  This product has not been evaluated or approved by the FDA or any other health authority for the diagnosis, treatment, cure, or prevention of any disease.

• Stability Disclaimer:
  Due to the inherent instability of NAD+, the seller guarantees purity and quantity at the time of shipment only. Proper handling, storage, and reconstitution by the researcher are essential for experimental validity.

• Researcher Responsibility:
  The purchaser assumes all responsibility for compliance with applicable regulations and for the safe, ethical, and appropriate use of this research compound.